Dr. Laura Ramos-Sepulveda

Dr. Laura Ramos-Sepulveda

Dr. Laura Ramos-Sepulveda

Assistant Professor, General Biology, Mycology.


laura.ramos-sepulveda@millersville.edu
Office: Caputo 306A
Phone: (717) 871-7431

Office Hours

M: 12:00 noon – 01:45 p.m.
T: 03:30 – 04:45 p.m.
W: 03:00 – 04:00 p.m.
R: 10:30 – 11:30 a.m.

Additional Information

Education:

B.S. in Horticulture, University of Puerto Rico - Mayaguez
Ph.D. in Plant Pathology, The Pennsylvania State University

Courses Taught:

BIOL 100 - General Biology and General Biology:Lab
BIOL 424 – Mycology

Areas of Specialization:

Molecular Biology (Bacteria and Fungi), Taxonomy (Bacteria), Microbe-Plant Interactions, Bacteria - Fungi Interactions

Research Interest(s):

Mushrooms are an ideal nutritional option for anyone; they are a good source of proteins, fibers, vitamins, and anti-oxidants. My current research interest is to study and stop Pseudomonas bacterium from causing bacterial blotch disease of mushrooms. The disease starts with brown spots around the mushroom cap and stalk, then the bacteria completely destroy the mushroom. It is believed that the Pseudomonas bacteria are always present inside the mushroom, waiting for the right temperature and humidity (environment factors) to attack. My approach to stop the bacteria is by studying the bacteria-fungi interactions and finding those crucial bacterial pathways that can be targeted (molecular biology). Since Pennsylvania is the main producer of mushrooms in the United States, this research is important because of the crop’s economic value. Mushrooms are the most economically important crop in the State.

Selected Publication(s):

Accepted Laboratory Guide Manual

2017.  Laboratory Guide for Identification of Plant Pathogenic Bacteria. 4th Edition. Editors J. B. Jones, G. Sundin, and S. A. Miller.  Author of two chapters, the use of rep-PCR and MLSA for identification, classification, and nomenclature of bacteria.

Accepted Article

Harmon C.L., Timilsina S., Jones D.D., Jeyaprakash A., Sun X., Vallad G.E., Ramos-Sepulveda L., Bull C.T., and Jones J.B. A New pathovar, Pseudomonas amygdali pv. loropetali pv. nov., proposed for the causal agent of gall disease of Loropetalum chinense. Phytopathology.

Published in Peer-Review Journals

2016. Koike S.T., Alger E.I., Ramos-Sepulveda L., and Bull C.T. First report of bacterial leaf spot caused by Pseudomonas syringae pv. tomato on kale in California. Plant Disease. http://dx.doi.org/10.1094/PDIS-10-16-1460-PDN

2015. Ramos L.S., Sinn J.P., Lehman B.L., Peter K.A., and McNellis T.W. Mutation of the Erwinia amylovora pyrC gene causes pyrimidine auxotrophy and attenuated virulence in apple trees and immature apple and pear fruits. Letters in Applied Microbiology 60: 572-579.

2014. Ramos L.S., Lehman B.L., Peter K.A., and McNellis T.W. Mutation of the Erwinia amylovora argD gene causes arginine auxotrophy, non-pathogenicity in apples, and reduced virulence in pears. Applied and Environmental Microbiology 80(21): 6739-6749.

2013. Ramos L.S., Lehman B.L., Sinn J.P., Pfeufer E.E., Halbrendt N.O., and McNellis T.W. The fire blight pathogen Erwinia amylovora requires the rpoN gene for pathogenicity in apple. Molecular Plant Pathology 14(8): 838-843.