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Millersville University Biology Department |
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Education:
B.A.,Wilson College
Ph.D., Bryn Mawr College
Courses Taught:
BIOL 100 -- General Biology
BIOL 205 -- Heredity and Human Affairs
BIOL 365 -- Genetics
BIOL 462 -- Molecular Biology
BIOL 466 -- Cell and Molecular Techniques
Areas of Specialization:
Genetics, cell and molecular biology
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Research Interest(s):
My research (done in collaboration with Dr. Ralph
Keil, Hershey Medical Center) focuses on a gene named
DEG1 that is involved in mitotic recombination in yeast
(Saccharomyces cerevisiae). Using molecular biology
techniques, my students and I successfully cloned this
gene and developed yeast strains with diverse forms
of DEG1. We are evaluating strains with DEG1 deletions,
point mutations, and excess amounts of the DEG1 gene
product to determine how these changes impact recombination
rates, resistance to elevated temperatures, levels of
RNA transcripts, and pseudouridyl synthetase activity.
This analysis should provide insight into the mechanisms
and pathways involved in recombination, DNA repair and
genome maintenance. Student participants gain valuable
experience in the scientific process, in genetic analysis,
and in techniques such as PCR, restriction mapping,
bacterial and yeast transformation, in vitro mutagenesis,
and phenotype screening.
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Selected Publication(s):
1999.
Effects of DEG1 mutations on HOT1-dependent genetic
recombination in Saccharomyces cerevisiae do not result
solely from changes in transcription. Molecular Biology
of the Cell 10 (S) : 93a. (with H. Fogell, R. Prusty,
S. DiBartolomeis, and R. Keil).
1995. DNA Fingerprinting: Laboratory Exercise, Teacher's
Notes and Essay. Biotechnology Laboratory Manual,
Addison-Welsey Publishers. (with J. B. Piperberg and
G. M.Farganis).
1993.
Recovery of heterotrophic soil bacteria guilds from
transient gasoline pollution. Hazardous Waste and
Hazardous Materials. 3:267-273 (with K. Baker and
M. Diltz).
1993.
An introduction to DNA fingerprinting. American Biology
Teacher 55: 216-221. (with J. Piperberg and G. Farganis).
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