Publications

Changes in Larval Mosquito Microbiota Reveal Non-target Effects of Insecticide Treatments in Hurricane-Created Habitats

Faculty Author(s): Wallace, John R.
Student Author(s): -
Department: BIOL
Publication: Microbial Ecology
Year: 2018
Abstract: To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s00248-018-1175-3 Byline: Joseph P. Receveur (1,2), Jennifer L. Pechal (2), M. Eric Benbow (2,3,4), Gary Donato (5), Tadhgh Rainey (5), John R. Wallace (1) Keywords: Microbial ecology; Microbe; Higher organism interactions; Community genomics; Non-target effects Abstract: Ephemeral aquatic habitats and their associated microbial communities (microbiomes) play important roles in the growth and development of numerous aquatic insects, including mosquitoes (Diptera). Biological control agents, such as Bacillus thuringiensis israelensis (Bti) or insect growth regulators (e.g., methoprene), are commonly used to control mosquitoes in these habitats. However, it is unknown how commonly used control compounds affect the mosquito internal microbiome and potentially alter their life history traits. The objectives of this study were threefold: characterize the internal microbiota of Aedes larvae (Culicidae) in ephemeral forested mosquito habitat using high-throughput amplicon based sequencing, assess how mosquito control treatments affect the internal microbial communities of larval mosquitoes, and determine if changes to the microbiome resulted from direct or indirect treatment effects. The larval microbiome varied in community composition and diversity with development stage and treatment, suggesting potential effects of control compounds on insect microbial ecology. While microbial community differences due to Bti treatment were a result of indirect effects on larval development, methoprene had significant impacts on bacterial and algal taxa that could not be explained by indirect treatment effects. These results provide new information on the interactions between pesticide treatments and insect microbial communities. Author Affiliation: (1) 0000 0001 1534 1738, grid.260049.9, Department of Biology, Millersville University, Millersville, PA, USA (2) 0000 0001 2150 1785, grid.17088.36, Department of Entomology, Michigan State University, East Lansing, MI, USA (3) 0000 0001 2150 1785, grid.17088.36, Department of Osteopathic Medical Specialties, Michigan State University, East Lansing, MI, USA (4) 0000 0001 2150 1785, grid.17088.36, Ecology, Evolutionary Biology and Behavior Program, Michigan State University, East Lansing, MI, USA (5) Hunterdon County Division of Health, Flemington, NJ, USA Article History: Registration Date: 06/03/2018 Received Date: 04/10/2017 Accepted Date: 06/03/2018 Online Date: 17/03/2018 Article note: Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00248-018-1175-3) contains supplementary material, which is available to authorized users.
Link: Changes in Larval Mosquito Microbiota Reveal Non-target Effects of Insecticide Treatments in Hurricane-Created Habitats